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Sangon Biotech perlprimer software
Perlprimer Software, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/perlprimer+software/perlprimer+software/pm33206273-90-4-10
Average 90 stars, based on 1 article reviews
perlprimer software - by Bioz Stars, 2026-10
90/100 stars

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Article Title: Myricanol 5-fluorobenzyloxy ether regulation of survivin pathway inhibits human lung adenocarcinoma A549 cells growth in vitro
Article Snippet: Reverse transcription was performed using a PrimeScriptTM RT reagent Kit (TaKaRa, Japan), and 1 μg RNA was reverse transcribed into cDNA in a 25 μL reaction system and stored at − 80 °C until use. .. All oligonucleotide primers were designed using the Perlprimer software and synthesized commercially (Sangon Biotechnology, Shanghai, China). .. The sequence of the primers is as follow: 5′-GGTCTCCTCTGACTTCAACA-3′ (forward) and 5′-AGCCAAATTCGTTGTCATAC-3′ (reverse) for GAPDH, 5′-TCTGGAGGATTTGGTGATGTC-3′ (forward) and 5′-CATTTTCTTGGCATCAGGTC-3′ (reverse) for caspase-9,5′-TTAGCAGCGGAACAAGGAGT-3′ (forward) and 5′-AGAAACGGGAACCAGGACAC-3′ (reverse) for p21, 5′-AAGCTGAGCGAGTGTCTCAAG-3′ (forward) and 5′-CAAAGTAGAAAAGGGCGACAAC-3′ (reverse) for Bax, 5′-ATGAAGTGAAGGCCATGATTG-3′ (forward) and 5′-TCCTTTAACGATGTCCACCAG-3′ (reverse) for PARP, 5′-CCCACTGAGAACGAGCCAGA-3′ (forward) and 5′-AAAGGAAAGCGCAACCGGAC-3′ (reverse) for survivin, 5′-ATGTGTGTGGAGAGCGTCAAC-3′ (forward) and 5′-AGAGACAGCCAGGAGAAATCAAAC-3′ (reverse) for Bcl-2. qPCR was performed on the StepOnePlus (Applied Biosystems, USA) using a SYBR Premix EX TaqTM II (TaKaRa, Japan) following the manufacturer’s protocol.

Article Title: Myricanol Induces Apoptotic Cell Death and Anti-Tumor Activity in Non-Small Cell Lung Carcinoma in Vivo
Article Snippet: .. All of the oligonucleotide primers were designed using PerlPrimer software and synthesized commercially (Sangon Biotechnology, Shanghai, China). .. The sequences of the primers are as follows: 5'-GGTCTCCTCTGACTTCAACA-3' (forward) and 5'-AGCCAAATTCGTTGTCATAC-3' (reverse) for GAPDH, 5'-AAGCTGAGCGAG TGTCTCAAG-3' (forward) and 5'-CAAAGTAGAAAAGGGCGACAAC-3' (reverse) for Bax,5'-ATGTGTGTGGAGAGCGTCAAC-3' (forward) and 5'-AGAGACAGCCAGGAGAAATCAAAC-3' (reverse) for Bcl-2, 5'-TGACGGACAGACAGACAGACACC-3' (forward) and 5'-AGAACAGCCCAGAAGTTGGACGA-3' (reverse) for VEGF, 5'-AGCCAGACGATCATGCAGCTACTA-3' (forward) and 5'-TGTGGTAAT CCACTTTCATCCATTG-3' (reverse) for HIF-1α, and 5'-AGGTCATCTCGGCTGTTCC TG-3' (forward) and 5'-TCATCCTCACTGCGGCTGTC-3' (reverse) for survivin. qRT-PCR was performed on 7500 StepOnePlusTM (Applied Biosystems, Foster, CA, USA) for 40 cycles using SYBR premix EX Taq (TaKaRa, Dalian, China) in accordance with the manufacturer’s protocol.

Article Title: Anti-inflammatory effects and mechanism of the total flavonoids from Artemisia scoparia Waldst. et kit. in vitro and in vivo.
Article Snippet: Artemisia scoparia Waldst. et Kit. is traditionally used for the treatment of jaundice urinary retention, itching wet sores, infectious icteric hepatitis and influenza in Uighur medicine.. This study aimed to further illuminate the anti-inflammatory effects and mechanism of the total flavonoids (ASTF) from Artemisia scoparia Waldst. et Kit.. In vitro, RAW 264.7 cells were pretreated with ASTF 1 h before stimulation with LPS (1 μg/mL) for 24 h. Then, the concentrations of NO, PGE2, TNF-α, IL-6 and MCP-1 in the medium were determined.

Article Title: Thalidomide Inhibits Angiogenesis via Downregulation of VEGF and Angiopoietin-2 in Crohn's Disease.
Article Snippet: Immune-mediated angiogenesis is important in the pathogenesis of inflammatory bowel disease and targeted treatment could alleviate the disease.. Thalidomide is an effective drug in inflammatory bowel disease, which might be related to its multiple role in anti-inflammatory, immunoregulatory, and anti-angiogenesis.. This study is to investigate the effect of thalidomide on angiogenesis in tissues from patients and in vitro cells.

Article Title: Growth-inhibiting and apoptosis-inducing activities of Myricanol from the bark of Myrica rubra in human lung adenocarcinoma A549 cells.
Article Snippet: Myrica rubra (Lour.). Sieb.. Et Zucc. is a myricaceae Myrica plant.

Synthesized:

Article Title: Myricanol 5-fluorobenzyloxy ether regulation of survivin pathway inhibits human lung adenocarcinoma A549 cells growth in vitro
Article Snippet: Reverse transcription was performed using a PrimeScriptTM RT reagent Kit (TaKaRa, Japan), and 1 μg RNA was reverse transcribed into cDNA in a 25 μL reaction system and stored at − 80 °C until use. .. All oligonucleotide primers were designed using the Perlprimer software and synthesized commercially (Sangon Biotechnology, Shanghai, China). .. The sequence of the primers is as follow: 5′-GGTCTCCTCTGACTTCAACA-3′ (forward) and 5′-AGCCAAATTCGTTGTCATAC-3′ (reverse) for GAPDH, 5′-TCTGGAGGATTTGGTGATGTC-3′ (forward) and 5′-CATTTTCTTGGCATCAGGTC-3′ (reverse) for caspase-9,5′-TTAGCAGCGGAACAAGGAGT-3′ (forward) and 5′-AGAAACGGGAACCAGGACAC-3′ (reverse) for p21, 5′-AAGCTGAGCGAGTGTCTCAAG-3′ (forward) and 5′-CAAAGTAGAAAAGGGCGACAAC-3′ (reverse) for Bax, 5′-ATGAAGTGAAGGCCATGATTG-3′ (forward) and 5′-TCCTTTAACGATGTCCACCAG-3′ (reverse) for PARP, 5′-CCCACTGAGAACGAGCCAGA-3′ (forward) and 5′-AAAGGAAAGCGCAACCGGAC-3′ (reverse) for survivin, 5′-ATGTGTGTGGAGAGCGTCAAC-3′ (forward) and 5′-AGAGACAGCCAGGAGAAATCAAAC-3′ (reverse) for Bcl-2. qPCR was performed on the StepOnePlus (Applied Biosystems, USA) using a SYBR Premix EX TaqTM II (TaKaRa, Japan) following the manufacturer’s protocol.

Article Title: Myricanol Induces Apoptotic Cell Death and Anti-Tumor Activity in Non-Small Cell Lung Carcinoma in Vivo
Article Snippet: .. All of the oligonucleotide primers were designed using PerlPrimer software and synthesized commercially (Sangon Biotechnology, Shanghai, China). .. The sequences of the primers are as follows: 5'-GGTCTCCTCTGACTTCAACA-3' (forward) and 5'-AGCCAAATTCGTTGTCATAC-3' (reverse) for GAPDH, 5'-AAGCTGAGCGAG TGTCTCAAG-3' (forward) and 5'-CAAAGTAGAAAAGGGCGACAAC-3' (reverse) for Bax,5'-ATGTGTGTGGAGAGCGTCAAC-3' (forward) and 5'-AGAGACAGCCAGGAGAAATCAAAC-3' (reverse) for Bcl-2, 5'-TGACGGACAGACAGACAGACACC-3' (forward) and 5'-AGAACAGCCCAGAAGTTGGACGA-3' (reverse) for VEGF, 5'-AGCCAGACGATCATGCAGCTACTA-3' (forward) and 5'-TGTGGTAAT CCACTTTCATCCATTG-3' (reverse) for HIF-1α, and 5'-AGGTCATCTCGGCTGTTCC TG-3' (forward) and 5'-TCATCCTCACTGCGGCTGTC-3' (reverse) for survivin. qRT-PCR was performed on 7500 StepOnePlusTM (Applied Biosystems, Foster, CA, USA) for 40 cycles using SYBR premix EX Taq (TaKaRa, Dalian, China) in accordance with the manufacturer’s protocol.

Article Title: Anti-inflammatory effects and mechanism of the total flavonoids from Artemisia scoparia Waldst. et kit. in vitro and in vivo.
Article Snippet: Artemisia scoparia Waldst. et Kit. is traditionally used for the treatment of jaundice urinary retention, itching wet sores, infectious icteric hepatitis and influenza in Uighur medicine.. This study aimed to further illuminate the anti-inflammatory effects and mechanism of the total flavonoids (ASTF) from Artemisia scoparia Waldst. et Kit.. In vitro, RAW 264.7 cells were pretreated with ASTF 1 h before stimulation with LPS (1 μg/mL) for 24 h. Then, the concentrations of NO, PGE2, TNF-α, IL-6 and MCP-1 in the medium were determined.

Article Title: Thalidomide Inhibits Angiogenesis via Downregulation of VEGF and Angiopoietin-2 in Crohn's Disease.
Article Snippet: Immune-mediated angiogenesis is important in the pathogenesis of inflammatory bowel disease and targeted treatment could alleviate the disease.. Thalidomide is an effective drug in inflammatory bowel disease, which might be related to its multiple role in anti-inflammatory, immunoregulatory, and anti-angiogenesis.. This study is to investigate the effect of thalidomide on angiogenesis in tissues from patients and in vitro cells.

Article Title: Growth-inhibiting and apoptosis-inducing activities of Myricanol from the bark of Myrica rubra in human lung adenocarcinoma A549 cells.
Article Snippet: Myrica rubra (Lour.). Sieb.. Et Zucc. is a myricaceae Myrica plant.



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